Reporter

Part:BBa_K1459017:Design

Designed by: Grzegorz Ścibisz   Group: iGEM14_Warsaw   (2014-10-17)


pmrC-GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 25
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 696


Design Notes

Apart from amplifying the sequence of interest we had to also perform a directed mutagenesis to eradicate XbaI site from pmrC sequence.


Source

We obtained pmrC-GFP sequence thanks to kindness of prof. He from the University of Chicago.

References